📚 Syllabus & Course Curriculum
Bioinformatics & Computational Biology
Module-by-module breakdown of Wastewater & Environmental AMR Surveillance Using Metagenomics, from foundations to a certified capstone project.
Rationale
• One catchment sample as a proxy for community-level resistance carriage
• What wastewater surveillance detects that clinical isolate reporting misses
• Ethical advantage of a non-individual sample, and its interpretive cost
Sampling
• Grab against composite sampling and the diurnal variation that decides it
• Influent, effluent, sludge and receiving water as different questions
• Cold chain, preservation and hold times before nucleic acid degrades
Laboratory
• Concentration methods and their differing recovery efficiencies
• Inhibitor removal from a matrix that is rich in humics and particulates
• Process and extraction controls needed to interpret a negative result
Analysis
• Normalisation to 16S rRNA or bacterial cell number for cross-site comparison
• Flow and population normalisation before any temporal trend is claimed
• Detection limits and the reality that absence is usually undersampling
Public Health
• Building a time series and separating a real signal from rainfall dilution
• Global initiatives and the WHO Tricycle protocol as a comparability framework
• Reporting to public health authorities and the risk of over-claiming from one site
Earn government-registered certification in Wastewater & Environmental AMR Surveillance Using Metagenomics
e-Certificate and e-Marksheet issued on successful completion.
