Programme Parameters
Foundation
3 Days (4.5 Hrs)
Online (e-LMS)
• No prior experience required — basic computer literacy is sufficient.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
This 3-day intensive course introduces participants to antimicrobial resistance (AMR) surveillance using wastewater metagenomics. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
This 3-day intensive course introduces participants to antimicrobial resistance (AMR) surveillance using wastewater metagenomics.
📋 Course Objectives
1. Put biotechnology techniques to work on real datasets and case studies.
2. Assemble a documented case study that evidences your applied capability.
👥 Who Should Enroll?
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
🚀 Key Learning Outcomes
• A portfolio-grade biotechnology deliverable you can defend and extend.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Wastewater as a Population Sample
• One catchment sample as a proxy for community-level resistance carriage
• What wastewater surveillance detects that clinical isolate reporting misses
• Ethical advantage of a non-individual sample, and its interpretive cost
Field Design and Collection
• Grab against composite sampling and the diurnal variation that decides it
• Influent, effluent, sludge and receiving water as different questions
• Cold chain, preservation and hold times before nucleic acid degrades
Concentration and Extraction
• Concentration methods and their differing recovery efficiencies
• Inhibitor removal from a matrix that is rich in humics and particulates
• Process and extraction controls needed to interpret a negative result
Quantifying the Environmental Resistome
• Normalisation to 16S rRNA or bacterial cell number for cross-site comparison
• Flow and population normalisation before any temporal trend is claimed
• Detection limits and the reality that absence is usually undersampling
Surveillance in Practice
• Building a time series and separating a real signal from rainfall dilution
• Global initiatives and the WHO Tricycle protocol as a comparability framework
• Reporting to public health authorities and the risk of over-claiming from one site
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | BWA |
| Covered Tool / Platform | SAMtools |
| Covered Tool / Platform | GATK |
| Covered Tool / Platform | FastQC |
| Covered Tool / Platform | Trimmomatic |
| Covered Tool / Platform | R/Bioconductor |
| Covered Tool / Platform | IGV |
| Covered Tool / Platform | PLINK |
Programme Faculty & Mentors
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