๐ Syllabus & Course Curriculum
Data Science & Analytics
Module-by-module breakdown of siRNA-Based Gene Silencing, from foundations to a certified capstone project.
Outline
Explore the foundational concepts of RNA interference (RNAi) and small interfering RNAs (siRNA). โข Identify key applications of siRNA in therapeutics and gene regulation. โข Understand the basic structure and functional rules governing siRNAs.
Outline
Distinguish between siRNA, microRNA (miRNA), and short hairpin RNA (shRNA) based on structure and mechanism. โข Analyze the unique characteristics and roles of each RNAi molecule. โข Discuss potential off-target effects and the importance of GC content in siRNA design.
Outline
Introduce the principles of RNA secondary structures, including base pairing, stem-loops, and hairpins. โข Interpret different RNA structure formats. โข Lay the groundwork for advanced RNA folding analysis.
Outline
Utilize leading RNA secondary structure prediction tools such as RNAfold (ViennaRNA), Mfold, and RNAstructure. โข Practice interpreting Free Energy (ฮG) values to assess RNA stability. โข Generate and analyze structure ensembles for comprehensive RNA folding insights.
Outline
Apply theoretical knowledge to hands-on siRNA sequence design exercises. โข Leverage professional bioinformatics platforms like Genscript for effective siRNA creation. โข Evaluate designed siRNA sequences for optimal efficiency and specificity.
Earn government-registered certification in siRNA-Based Gene Silencing
e-Certificate and e-Marksheet issued on successful completion.
