Programme Parameters
Graduate / Intermediate
4 Weeks (40 Hrs)
Online (e-LMS)
• A basic understanding of the subject area and fundamental programming or scientific concepts.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
CRISPR-Cas12/Cas13 for Rapid Pathogen Detection explores how genome-editing enzymes became the basis of a new generation of diagnostics. You learn the distinctive collateral-cleavage activity of Cas12 and Cas13 that makes them exquisite nucleic-acid detectors, and how platforms such as DETECTR and SHERLOCK combine them with amplification for rapid, sensitive, field-deployable tests. The course covers assay design, readout methods, and the role these tools played in outbreak response. You finish able to understand and reason about designing a CRISPR-based diagnostic. A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
This course covers CRISPR-based diagnostics — how Cas12 and Cas13 enzymes power rapid, sensitive nucleic-acid detection platforms like DETECTR and SHERLOCK for pathogens.
📋 Course Objectives
1. Explain Cas12 and Cas13 collateral-cleavage detection.
2. Describe the DETECTR and SHERLOCK platforms.
3. Combine CRISPR detection with nucleic-acid amplification.
4. Design assay readouts for sensitivity and speed.
5. Assess CRISPR diagnostics for field and outbreak use.
👥 Who Should Enroll?
• Molecular diagnostics and biotech researchers
• Microbiology and infectious-disease scientists
• Point-of-care and public-health professionals
• Students specialising in diagnostics
🚀 Key Learning Outcomes
• An understanding of CRISPR-based diagnostics.
• The ability to reason about assay design.
• A foundation in point-of-care molecular testing.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Foundations of CRISPR-Cas12/Cas13 for Rapid Pathogen Detection and Core Biological Principles
Analyze the molecular mechanisms underlying CRISPR-Cas12/Cas13 systems and their applications in rapid pathogen detection • Develop a comprehensive understanding of the core biological principles governing CRISPR-Cas12/Cas13 function and specificity • Evaluate the current state of CRISPR-Cas12/Cas13 technology and its potential for rapid pathogen detection in various fields
Laboratory Techniques, Protocols, and Data Collection
Configure and optimize laboratory protocols for CRISPR-Cas12/Cas13-based pathogen detection, including sample preparation and data collection • Implement quality control measures to ensure the accuracy and reliability of CRISPR-Cas12/Cas13-based detection assays • Design and conduct experiments to validate the performance of CRISPR-Cas12/Cas13-based detection systems
Bioinformatics Tools and Computational Analysis
Apply bioinformatics tools and computational methods to analyze and interpret CRISPR-Cas12/Cas13-based sequencing data • Develop and implement computational pipelines to process and analyze large-scale CRISPR-Cas12/Cas13-based sequencing datasets • Evaluate the performance of different bioinformatics tools and computational methods for CRISPR-Cas12/Cas13-based data analysis
Research Methodology and Experimental Design
Design and develop research studies to investigate the applications and limitations of CRISPR-Cas12/Cas13-based pathogen detection • Implement robust experimental designs and statistical analysis methods to validate the results of CRISPR-Cas12/Cas13-based detection assays • Analyze and interpret the results of CRISPR-Cas12/Cas13-based research studies and develop conclusions based on the data
Advanced CRISPR-Cas12/Cas13 for Rapid Pathogen Detection Applications and Translational Research
Investigate the applications of CRISPR-Cas12/Cas13-based pathogen detection in various fields, including clinical diagnostics and public health • Develop and evaluate novel CRISPR-Cas12/Cas13-based detection systems for emerging and re-emerging pathogens • Design and conduct translational research studies to bridge the gap between CRISPR-Cas12/Cas13-based research and clinical applications
Regulatory Compliance, Bioethics, and Safety Standards
Evaluate the regulatory frameworks and guidelines governing the use of CRISPR-Cas12/Cas13-based pathogen detection systems • Develop and implement bioethics and safety protocols to ensure the responsible use of CRISPR-Cas12/Cas13-based detection systems • Analyze the potential risks and benefits associated with the use of CRISPR-Cas12/Cas13-based detection systems and develop strategies to mitigate risks
Industry Applications, Career Pathways, and Case Studies
Investigate the current and future industry applications of CRISPR-Cas12/Cas13-based pathogen detection, including clinical diagnostics and biotechnology • Develop a comprehensive understanding of the career pathways and professional opportunities available in the field of CRISPR-Cas12/Cas13-based pathogen detection • Analyze case studies of successful CRISPR-Cas12/Cas13-based detection systems and develop insights into the key factors contributing to their success
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | Python |
| Covered Tool / Platform | TensorFlow |
| Covered Tool / Platform | CRISPR-Cas12/Cas13 software |
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