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DSTC-110753 Online (e-LMS) Foundation

NGS Data Processing and Interpretation

by - DSTC

Master NGS Data Processing and Interpretation in 4 weeks through hands-on, project-based online training with DSTC.

★★★★★ Be the first to review 4 Weeks · 40 hrs e-Certificate Included
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From ₹5,000 + GST

Programme Parameters

Educational Level:
Foundation
Duration & Workload:
4 Weeks (40 Hrs)
Delivery Mode:
Online (e-LMS)
Prerequisites:
• No prior experience required — basic computer literacy is sufficient.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.

About This Course

The NGS Data Processing and Interpretation Internship is designed to introduce learners to the basic concepts of Next-Generation Sequencing and its role in modern biological research. This internship helps participants understand how sequencing data is generated, processed, checked for quality, and interpreted for research applications. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.

🎯 Program Aim

The NGS Data Processing and Interpretation Internship is designed to introduce learners to the basic concepts of Next-Generation Sequencing and its role in modern biological research. This internship helps participants understand how sequencing data is generated, processed, checked for quality, and interpreted for research applications.

📋 Course Objectives

1. Translate biotechnology theory into practical, reproducible analysis.
2. Assemble a documented case study that evidences your applied capability.

👥 Who Should Enroll?

• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology

🚀 Key Learning Outcomes

• Tangible, reproducible biotechnology work to show supervisors or employers.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.

💎 What You'll Gain

🎥

Live & Recorded Sessions

Lifetime access to class recordings
🎓

e-Certificate on Completion

Cryptographically verified credential
💬

Post-Programme Support

Direct access to mentors & council
💻

Hands-On Experience

Notebooks, real-world code & datasets

Curriculum Outline

Module 1 Outline

Introduction to NGS

What is Next-Generation Sequencing? • Difference between Sanger sequencing and NGS • Common NGS platforms: Illumina, Ion Torrent, PacBio, Oxford Nanopore • Applications of NGS in health, agriculture, microbiology, and biotechnology • Basic types of NGS studies: DNA-Seq, RNA-Seq, WGS, targeted sequencing, and metagenomics

Module 2 Outline

Understanding NGS Data

What are sequencing reads? • Basic NGS file formats: FASTQ, FASTA, SAM, BAM, VCF, GTF/GFF • Introduction to read quality • Phred quality score in simple terms • Read length, depth, and coverage • Why good-quality data is important

Module 3 Outline

Quality Check and Data Cleaning

Why quality control is needed in NGS • Introduction to FastQC reports • Common quality issues in sequencing data • Adapter contamination and low-quality reads • Basics of read trimming and filtering • Introduction to tools like FastQC, MultiQC, Trimmomatic, Cutadapt, and fastp

Module 4 Outline

Basic NGS Analysis and Reporting

Overview of a simple NGS analysis workflow • Introduction to read alignment and genome mapping • Basic idea of SAM/BAM files • Introduction to variant calling and VCF files • Basic idea of RNA-Seq count data and gene expression • Introduction to biological interpretation • Preparing a simple NGS analysis summary report

Technical Specifications

ParameterRequirement
Covered Tool / PlatformNext-Generation Sequencing
Covered Tool / PlatformFASTQ and FASTA Files
Covered Tool / PlatformSAM and BAM Files
Covered Tool / PlatformVCF Files
Covered Tool / PlatformGTF/GFF Annotation Files
Covered Tool / PlatformPhred Quality Score
Covered Tool / PlatformRead Length, Depth, and Coverage
Covered Tool / PlatformFastQC
Covered Tool / PlatformMultiQC
Covered Tool / PlatformTrimmomatic
Covered Tool / PlatformCutadapt
Covered Tool / Platformfastp
Covered Tool / PlatformSAMtools Awareness
Covered Tool / PlatformIGV Genome Browser Awareness
Covered Tool / PlatformGoogle Colab
Covered Tool / PlatformLinux Terminal Awareness
Covered Tool / PlatformExcel / Google Sheets

Frequently Asked Questions

This internship focuses on the basic understanding of Next-Generation Sequencing data, file formats, quality control, preprocessing, alignment concepts, variant interpretation, RNA-Seq data, and biological reporting.

Yes. The internship is designed for beginners and early-stage learners who want to understand NGS data analysis in a simple and structured way.

You will learn the basics of NGS, sequencing reads, FASTQ files, quality scores, QC reports, read cleaning, SAM/BAM files, VCF interpretation, RNA-Seq count data, and NGS report preparation.

Coding knowledge is helpful but not compulsory. The internship introduces Google Colab, Linux terminal awareness, and basic tool concepts in a beginner-friendly manner.

The internship introduces tools and concepts such as FastQC, MultiQC, Trimmomatic, Cutadapt, fastp, SAMtools awareness, IGV Genome Browser awareness, Google Colab, and Excel / Google Sheets.

NGS data processing is important because raw sequencing data must be checked, cleaned, organized, and interpreted before it can be used for biological or research conclusions.

Students, researchers, PhD scholars, faculty members, biotechnology learners, bioinformatics beginners, laboratory professionals, and life science learners can apply.

Learning NGS data interpretation can support career growth in genomics research, bioinformatics, biotechnology, clinical research, microbiology, agriculture, and molecular biology laboratories.

Yes. The internship helps learners understand how NGS results are interpreted and summarized, which can support academic projects, research reports, thesis work, and technical presentations.

Yes. Participants receive e-Certification + e-Marksheet upon successful completion of the internship. The NGS Data Processing and Interpretation Internship helps learners build a strong beginner-level foundation in sequencing data, quality control, preprocessing, alignment concepts, variant interpretation, RNA-Seq data, and biological reporting. It is a suitable starting point for learners who want to enter genomics, bioinformatics, biotechnology, and life science data analysis.

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