Master Flow Cytometry & Single-Cell Analysis: From Sample to Data in 4 weeks through hands-on, project-based online training with DSTC.
Flow cytometry is a cornerstone technology in immunology, cancer biology, hematology, and cell therapy, enabling rapid multiparametric analysis of thousands of cells per second. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
Flow cytometry is a cornerstone technology in immunology, cancer biology, hematology, and cell therapy, enabling rapid multiparametric analysis of thousands of cells per second.
1. Apply biotechnology methods to authentic research and industry problems.
2. Produce a reproducible, portfolio-ready project you can cite in a thesis, paper, or job application.
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
• Tangible, reproducible biotechnology work to show supervisors or employers.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
Understand flow cytometry principles, FSC/SSC and fluorescence channels • Perform cell washing, counting, and concentration adjustment • Execute staining protocols with fluorochrome‑tagged antibodies
Configure voltages, fluidics and select optimal parameters • Run controls and stained samples on the cytometer • Apply live gating, compensation and export gated data
Design simple panels, match markers to fluorochromes and minimize spillover • Calculate population percentages, MFI and compare experimental conditions • Generate publication‑ready histograms, dot plots and explore t‑SNE/UMAP concepts
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | Flow cytometer |
| Covered Tool / Platform | FlowJo |
| Covered Tool / Platform | Cytobank |
| Covered Tool / Platform | FCS files |
| Covered Tool / Platform | t-SNE |
| Covered Tool / Platform | UMAP |
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