📚 Syllabus & Course Curriculum
Biotechnology & Genetic Engineering
Module-by-module breakdown of Hands-on Course on Mammalian Cell Culture & Aseptic Techniques, from foundations to a certified capstone project.
Facility
• Biosafety levels, cabinet classes and correct airflow practice
• Aseptic technique: the specific movements that cause most contamination
• Cleaning, disinfection and incubator maintenance schedules
Culture
• Media, serum and supplement selection, and lot-to-lot variability
• Thawing, passaging and split ratios for adherent and suspension cells
• Growth curves, confluence judgement and avoiding over-passaging
Quality
• Bacterial, fungal and mycoplasma contamination: detection and response
• Cell line authentication by STR profiling and the misidentification literature
• Cryopreservation, bank structure and recovery viability
Assays
• Viability and proliferation assays and their interference artefacts
• Transfection and treatment protocols with appropriate controls
• Imaging and counting, manual versus automated, and their disagreement
Practice
• Records that make an experiment repeatable months later
• Waste handling and regulatory compliance for biological material
• Troubleshooting: diagnosing poor growth from evidence rather than guesswork
Earn government-registered certification in Hands-on Course on Mammalian Cell Culture & Aseptic Techniques
e-Certificate and e-Marksheet issued on successful completion.
