Programme Parameters
Graduate / Intermediate
3 Days (4.5 Hrs)
Online (e-LMS)
• A basic understanding of the subject area and fundamental programming or scientific concepts.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
Mammalian cell culture is a core technique in modern biological sciences, playing an essential role in drug discovery, cancer research, toxicology, vaccine development, and regenerative medicine. Proper aseptic handling, contamination control, and understanding cell behavior are crucial for generating reproducible and biologically meaningful results. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
Mammalian cell culture is a core technique in modern biological sciences, playing an essential role in drug discovery, cancer research, toxicology, vaccine development, and regenerative medicine. Proper aseptic handling, contamination control, and understanding cell behavior are crucial for generating reproducible and biologically meaningful results.
📋 Course Objectives
1. Put biotechnology techniques to work on real datasets and case studies.
2. Build a defensible project you can showcase to supervisors, reviewers, or employers.
👥 Who Should Enroll?
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
🚀 Key Learning Outcomes
• A demonstrable biotechnology project for your research or industry portfolio.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Laboratory Setup and Sterility
• Biosafety levels, cabinet classes and correct airflow practice
• Aseptic technique: the specific movements that cause most contamination
• Cleaning, disinfection and incubator maintenance schedules
Establishing and Maintaining Lines
• Media, serum and supplement selection, and lot-to-lot variability
• Thawing, passaging and split ratios for adherent and suspension cells
• Growth curves, confluence judgement and avoiding over-passaging
Contamination and Cell Identity
• Bacterial, fungal and mycoplasma contamination: detection and response
• Cell line authentication by STR profiling and the misidentification literature
• Cryopreservation, bank structure and recovery viability
Working With Cultured Cells
• Viability and proliferation assays and their interference artefacts
• Transfection and treatment protocols with appropriate controls
• Imaging and counting, manual versus automated, and their disagreement
Documentation and Reproducibility
• Records that make an experiment repeatable months later
• Waste handling and regulatory compliance for biological material
• Troubleshooting: diagnosing poor growth from evidence rather than guesswork
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | Python |
| Covered Tool / Platform | Jupyter Notebook |
| Covered Tool / Platform | Google Colab |
| Covered Tool / Platform | Microsoft Excel |
| Covered Tool / Platform | Relevant Online Databases |
Frequently Asked Questions
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