Programme Parameters
Graduate / Intermediate
4 Weeks (40 Hrs)
Online (e-LMS)
• A basic understanding of the subject area and fundamental programming or scientific concepts.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
CRISPR-Cas9 Genome Editing takes you from the molecular mechanism of the Cas9–guide-RNA complex to designing and validating your own editing experiments. You will learn to select target sites, design and score guide RNAs, weigh delivery strategies, and distinguish knock-out from knock-in and base-editing approaches. A major focus is rigour: predicting and assaying off-target activity, choosing the right validation (sequencing, T7E1, ICE analysis), and interpreting outcomes honestly. The course connects the biology to practical workflow decisions so you can plan a defensible genome-editing study. A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
This course covers CRISPR-Cas9 genome editing from mechanism to bench practice: guide-RNA design, delivery, on- and off-target analysis, and experimental validation.
📋 Course Objectives
1. Explain the Cas9–gRNA mechanism and editing outcomes.
2. Design and score guide RNAs for a chosen target.
3. Compare delivery methods and editing strategies (KO, KI, base editing).
4. Predict and assay on- and off-target activity.
5. Choose validation methods and interpret editing results.
👥 Who Should Enroll?
• Molecular biology and genetics researchers
• PhD scholars and postdocs adopting genome editing
• Biotech R&D scientists
• Life-science students specialising in functional genomics
🚀 Key Learning Outcomes
• A complete, defensible CRISPR experiment design.
• The ability to design and evaluate guide RNAs.
• Confidence interpreting editing efficiency and specificity.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Foundations of CRISPR-Cas9 Genome Editing
Analyze the molecular mechanisms underlying CRISPR-Cas9 genome editing, including DNA targeting and cleavage • Design and optimize guide RNA sequences for efficient genome editing using bioinformatics tools • Evaluate the specificity and efficacy of CRISPR-Cas9 genome editing using various experimental approaches
Laboratory Techniques, Protocols, and Data Collection
Configure and operate laboratory equipment, including microscopes and PCR machines, for CRISPR-Cas9 experiments • Develop and implement standardized protocols for CRISPR-Cas9 genome editing, including DNA extraction and sequencing • Collect and analyze data from CRISPR-Cas9 experiments, including sequencing and phenotypic analysis
Bioinformatics Tools and Computational Analysis
Implement bioinformatics pipelines for analyzing CRISPR-Cas9 sequencing data, including read mapping and variant calling • Develop and apply computational models for predicting CRISPR-Cas9 off-target effects and optimizing genome editing • Analyze and visualize CRISPR-Cas9 genome editing data using various bioinformatics tools and programming languages
Research Methodology and Experimental Design
Design and conduct experiments to test hypotheses and evaluate the efficacy of CRISPR-Cas9 genome editing • Develop and implement controls and validation strategies for CRISPR-Cas9 experiments, including positive and negative controls • Evaluate and troubleshoot CRISPR-Cas9 experiments, including identifying and addressing common pitfalls and limitations
Advanced CRISPR-Cas9 Genome Editing Applications
Apply CRISPR-Cas9 genome editing to various biological systems, including mammalian cells and model organisms • Develop and implement novel CRISPR-Cas9 genome editing strategies, including base editing and prime editing • Evaluate the potential applications and limitations of CRISPR-Cas9 genome editing in various fields, including biotechnology and medicine
Regulatory Compliance, Bioethics, and Safety Standards
Evaluate the regulatory frameworks and guidelines governing CRISPR-Cas9 genome editing, including biosafety and biosecurity • Develop and implement strategies for ensuring compliance with regulatory requirements and safety standards • Analyze and discuss the ethical implications of CRISPR-Cas9 genome editing, including germline editing and gene drive
Industry Applications, Career Pathways, and Case Studies
Analyze the current and potential applications of CRISPR-Cas9 genome editing in various industries, including biotechnology and pharmaceuticals • Develop and implement career development strategies for professionals in the CRISPR-Cas9 genome editing field • Evaluate and discuss case studies of successful CRISPR-Cas9 genome editing applications, including therapeutic and agricultural applications
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | Python |
| Covered Tool / Platform | TensorFlow |
| Covered Tool / Platform | CRISPR-Cas9 software |
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