📚 Syllabus & Course Curriculum
Antimicrobial Resistance (AMR)
Module-by-module breakdown of Bacterial Gene Transfer: E. coli Plasmid Conjugation, Transformation & Antibiotic Marker Tracking, from foundations to a certified capstone project.
Biology
• Conjugation, natural transformation and transduction distinguished
• Plasmid replicons, incompatibility groups and copy number
• Mobilisable against self-transmissible plasmids and the tra machinery
Transformation
• Chemical competence with calcium chloride against electroporation
• Transformation efficiency calculation in cfu per microgram
• Controls that separate genuine transformants from contamination
Conjugation
• Donor, recipient and transconjugant strain design with distinct markers
• Filter and broth mating protocols and their differing efficiencies
• Counter-selection so that donors do not overgrow the plate
Tracking
• Antibiotic selection, working concentrations and breakdown over plate age
• Confirming transfer by plasmid extraction and PCR rather than growth alone
• Transfer frequency calculation and reporting it per recipient
Context
• Biosafety level, disposal and containment for antibiotic-resistant strains
• What laboratory mating frequencies do and do not say about environmental spread
• Linking the bench result to clinical resistance dissemination
Earn government-registered certification in Bacterial Gene Transfer: E. coli Plasmid Conjugation, Transformation & Antibiotic Marker Tracking
e-Certificate and e-Marksheet issued on successful completion.
