Programme Parameters
Foundation
3 Days (4.5 Hrs)
Online (e-LMS)
• No prior experience required — basic computer literacy is sufficient.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
This hands-on laboratory course focuses on understanding plasmid-mediated horizontal gene transfer in E. coli through transformation and conjugation experiments. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
This hands-on laboratory course focuses on understanding plasmid-mediated horizontal gene transfer in E. coli through transformation and conjugation experiments.
📋 Course Objectives
1. Master the fundamentals of conjugation experiments.
2. Apply biotechnology methods to authentic research and industry problems.
3. Assemble a documented case study that evidences your applied capability.
👥 Who Should Enroll?
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
• Data and computational scientists moving into conjugation experiments
🚀 Key Learning Outcomes
• Confidence to apply conjugation experiments in real projects.
• A demonstrable biotechnology project for your research or industry portfolio.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Mechanisms of Horizontal Transfer
• Conjugation, natural transformation and transduction distinguished
• Plasmid replicons, incompatibility groups and copy number
• Mobilisable against self-transmissible plasmids and the tra machinery
Getting DNA into E. coli
• Chemical competence with calcium chloride against electroporation
• Transformation efficiency calculation in cfu per microgram
• Controls that separate genuine transformants from contamination
Mating Experiments
• Donor, recipient and transconjugant strain design with distinct markers
• Filter and broth mating protocols and their differing efficiencies
• Counter-selection so that donors do not overgrow the plate
Following the Marker
• Antibiotic selection, working concentrations and breakdown over plate age
• Confirming transfer by plasmid extraction and PCR rather than growth alone
• Transfer frequency calculation and reporting it per recipient
Safety and Significance
• Biosafety level, disposal and containment for antibiotic-resistant strains
• What laboratory mating frequencies do and do not say about environmental spread
• Linking the bench result to clinical resistance dissemination
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | Arduino |
| Covered Tool / Platform | Raspberry Pi |
| Covered Tool / Platform | MQTT |
| Covered Tool / Platform | Node-RED |
| Covered Tool / Platform | ThingSpeak |
| Covered Tool / Platform | Python |
| Covered Tool / Platform | AWS IoT |
Frequently Asked Questions
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