Master NGS Data Processing and Interpretation in 4 weeks through hands-on, project-based online training with DSTC.
The NGS Data Processing and Interpretation Internship is designed to introduce learners to the basic concepts of Next-Generation Sequencing and its role in modern biological research. This internship helps participants understand how sequencing data is generated, processed, checked for quality, and interpreted for research applications. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
The NGS Data Processing and Interpretation Internship is designed to introduce learners to the basic concepts of Next-Generation Sequencing and its role in modern biological research. This internship helps participants understand how sequencing data is generated, processed, checked for quality, and interpreted for research applications.
1. Translate biotechnology theory into practical, reproducible analysis.
2. Assemble a documented case study that evidences your applied capability.
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
• Tangible, reproducible biotechnology work to show supervisors or employers.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
What is Next-Generation Sequencing? • Difference between Sanger sequencing and NGS • Common NGS platforms: Illumina, Ion Torrent, PacBio, Oxford Nanopore • Applications of NGS in health, agriculture, microbiology, and biotechnology • Basic types of NGS studies: DNA-Seq, RNA-Seq, WGS, targeted sequencing, and metagenomics
What are sequencing reads? • Basic NGS file formats: FASTQ, FASTA, SAM, BAM, VCF, GTF/GFF • Introduction to read quality • Phred quality score in simple terms • Read length, depth, and coverage • Why good-quality data is important
Why quality control is needed in NGS • Introduction to FastQC reports • Common quality issues in sequencing data • Adapter contamination and low-quality reads • Basics of read trimming and filtering • Introduction to tools like FastQC, MultiQC, Trimmomatic, Cutadapt, and fastp
Overview of a simple NGS analysis workflow • Introduction to read alignment and genome mapping • Basic idea of SAM/BAM files • Introduction to variant calling and VCF files • Basic idea of RNA-Seq count data and gene expression • Introduction to biological interpretation • Preparing a simple NGS analysis summary report
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | Next-Generation Sequencing |
| Covered Tool / Platform | FASTQ and FASTA Files |
| Covered Tool / Platform | SAM and BAM Files |
| Covered Tool / Platform | VCF Files |
| Covered Tool / Platform | GTF/GFF Annotation Files |
| Covered Tool / Platform | Phred Quality Score |
| Covered Tool / Platform | Read Length, Depth, and Coverage |
| Covered Tool / Platform | FastQC |
| Covered Tool / Platform | MultiQC |
| Covered Tool / Platform | Trimmomatic |
| Covered Tool / Platform | Cutadapt |
| Covered Tool / Platform | fastp |
| Covered Tool / Platform | SAMtools Awareness |
| Covered Tool / Platform | IGV Genome Browser Awareness |
| Covered Tool / Platform | Google Colab |
| Covered Tool / Platform | Linux Terminal Awareness |
| Covered Tool / Platform | Excel / Google Sheets |
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