📚 Syllabus & Course Curriculum
Data Science & Analytics
Module-by-module breakdown of RNA Interference (RNAi) and siRNA Techniques, from foundations to a certified capstone project.
Mechanism
• Dicer processing, RISC loading and Argonaute slicing
• siRNA, shRNA and miRNA distinguished by origin and by outcome
• Passenger strand selection and thermodynamic asymmetry
Design
• Design rules for potency and the algorithms that encode them
• Seed-region off-target effects as the dominant artefact in RNAi experiments
• Chemical modifications for stability and reduced immune stimulation
Delivery
• Lipid transfection, electroporation and viral shRNA vectors compared
• Transfection toxicity confounded with the phenotype being measured
• Hard-to-transfect primary cells and the resulting compromises
Controls
• Non-targeting controls, dose titration and knockdown quantification by RT-qPCR
• Protein-level confirmation by western blot — mRNA loss is not protein loss
• Rescue experiments as the strongest evidence of specificity
Therapeutics
• Approved siRNA drugs and the GalNAc conjugate delivery breakthrough
• Tissue targeting beyond the liver as the remaining barrier
• RNAi against CRISPR knockout: when transient knockdown is the better design
Earn government-registered certification in RNA Interference (RNAi) and siRNA Techniques
e-Certificate and e-Marksheet issued on successful completion.
