Programme Parameters
Graduate / Intermediate
3 Days (4.5 Hrs)
Online (e-LMS)
• A basic understanding of the subject area and fundamental programming or scientific concepts.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
Next-Generation Sequencing (NGS) has revolutionized the field of genomics by enabling the rapid sequencing of entire genomes and transcriptomes. This course offers an in-depth exploration of NGS data analysis using the Galaxy platform, a powerful and user-friendly web-based tool. Participants will learn to navigate the Galaxy interface, perform quality control, align sequences, and interpret results. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
Next-Generation Sequencing (NGS) has revolutionized the field of genomics by enabling the rapid sequencing of entire genomes and transcriptomes. This course offers an in-depth exploration of NGS data analysis using the Galaxy platform, a powerful and user-friendly web-based tool. Participants will learn to navigate the Galaxy interface, perform quality control, align sequences, and interpret results.
📋 Course Objectives
1. Translate biotechnology theory into practical, reproducible analysis.
2. Build a defensible project you can showcase to supervisors, reviewers, or employers.
👥 Who Should Enroll?
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
🚀 Key Learning Outcomes
• Tangible, reproducible biotechnology work to show supervisors or employers.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Foundations of NGS and Galaxy Platform
Master the fundamental principles and concepts of Next-Generation Sequencing technology • Navigate the Galaxy interface and configure user accounts for bioinformatics workflows • Execute quality control assessments and preprocessing steps for raw NGS data
Genome Assembly and Annotation
Utilize Galaxy tools for de novo and reference-guided genome assembly • Apply automated annotation pipelines to predict gene structures and functional elements • Validate assembly quality and assess completeness using benchmark metrics
Variant Identification and Analysis
Perform read mapping and alignment against reference genomes using industry-standard algorithms • Identify single nucleotide polymorphisms (SNPs) and structural variants from NGS data • Filter, annotate, and prioritize variants for clinical and research applications
RNA-Seq Analysis and Transcriptome Assembly
Process raw RNA-Seq reads using Hisat2 for accurate splice-aware alignment • Quantify gene and transcript abundance with FeatureCounts and related tools • Assemble transcriptomes and discover novel isoforms from sequencing data
Differential Gene Expression with DESeq2
Design robust differential expression experiments with proper biological replicates • Execute DESeq2 workflows within Galaxy to identify significantly regulated genes • Visualize expression patterns through volcano plots, heatmaps, and PCA analyses
Pathway Analysis and Functional Interpretation
Perform Gene Ontology (GO) enrichment and pathway over-representation analysis • Map differentially expressed genes to biological pathways and disease associations • Interpret multi-omics results to generate actionable biological hypotheses
Pipeline Construction and Workflow Optimization
Construct reproducible, automated analysis pipelines using Galaxy's workflow editor • Implement best practices and optimization strategies for efficient NGS data analysis • Customize parameters and troubleshoot common bioinformatics challenges
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | Galaxy Platform |
| Covered Tool / Platform | Hisat2 |
| Covered Tool / Platform | FeatureCounts |
| Covered Tool / Platform | DESeq2 |
| Covered Tool / Platform | FastQC |
| Covered Tool / Platform | MultiQC |
Frequently Asked Questions
Scholar Feedback & Reviews
Based on 0 scholar submissions
Rating Breakdown
No verified reviews published yet. Be the first to share your academic experience.
Leave Scholar Feedback
Your rating will help prospective scholars. Ratings below 3 stars are routed privately to the faculty mentor for immediate response.
