Programme Parameters
Graduate / Intermediate
3 Days (4.5 Hrs)
Online (e-LMS)
• A basic understanding of the subject area and fundamental programming or scientific concepts.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
Antimicrobial resistance and horizontal gene transfer are critical public health challenges, as they facilitate the emergence of multidrug-resistant pathogens. Metagenomics offers a powerful way to uncover resistance mechanisms and gene exchange within complex microbial ecosystems. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
Antimicrobial resistance and horizontal gene transfer are critical public health challenges, as they facilitate the emergence of multidrug-resistant pathogens. Metagenomics offers a powerful way to uncover resistance mechanisms and gene exchange within complex microbial ecosystems.
📋 Course Objectives
1. Put biotechnology techniques to work on real datasets and case studies.
2. Assemble a documented case study that evidences your applied capability.
👥 Who Should Enroll?
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
🚀 Key Learning Outcomes
• A portfolio-grade biotechnology deliverable you can defend and extend.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Resistance and Mobility
• Resistance mechanisms: efflux, target modification and enzymatic inactivation
• Conjugation, transformation and transduction as routes of gene movement
• Resistome and mobilome as distinct objects requiring distinct analysis
Data Generation and Preparation
• Shotgun metagenomics against amplicon approaches for resistance gene detection
• Read QC, host read removal and adapter handling with fastp and Bowtie2
• Depth requirements — low-abundance resistance genes are missed at shallow depth
Finding Resistance Genes
• CARD, ResFinder and AMRFinderPlus, and their differing curation philosophies
• Read-based against assembly-based detection and the sensitivity trade-off
• Identity and coverage thresholds and the false positives loose settings produce
Linking Genes to Hosts and Elements
• Assembly with metaSPAdes and binning with MetaBAT for genomic context
• Plasmid, integron and transposon identification with mobileOG and PlasmidFinder
• Host attribution and why short reads frequently cannot support the claim
What the Data Will and Will Not Support
• Gene presence is not resistance phenotype — the limits of genotypic prediction
• Evidence needed to argue a horizontal transfer event actually occurred
• Comparative and longitudinal designs for tracking resistome change
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | RStudio |
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