📚 Syllabus & Course Curriculum
Data Science & Analytics
Module-by-module breakdown of Intelligent Molecular Diagnostics & Primer Engineering, from foundations to a certified capstone project.
Chemistry
• Annealing temperature, magnesium concentration and their interaction
• Polymerase choice: proofreading, hot-start and processivity differences
• Reading a failed reaction from its amplification curve rather than guessing
Design
• Primer3 and NCBI Primer-BLAST, and the parameters that actually matter
• Tm matching, GC clamp, and self-dimer and hairpin prediction
• Specificity checking against the full genome, not only the target sequence
Optimisation
• Gradient PCR and touchdown protocols for stubborn primer pairs
• Additives — DMSO, betaine — for GC-rich and structured templates
• Contamination control and the physical separation of pre- and post-PCR work
Computation
• Learned models for primer performance and the small public training sets
• Automated design pipelines and the checks that must stay manual
• Amplification curve analysis and automated call quality flags
Application
• Multiplex panel design and the combinatorial dimer problem
• Positive, negative and internal amplification controls as non-negotiable
• Limit of detection determination and honest reporting of assay range
Earn government-registered certification in Intelligent Molecular Diagnostics & Primer Engineering
e-Certificate and e-Marksheet issued on successful completion.
