Master Hands-on Introduction to NGS Data Analysis: From Raw Sequencing Reads to Biological Interpretation in 4 weeks through hands-on, project-based online training with DSTC.
Data Science & Analytics
Module-by-module breakdown of Hands-on Introduction to NGS Data Analysis: From Raw Sequencing Reads to Biological Interpretation, from foundations to a certified capstone project.
Outline
Explore major NGS platforms and applications • Identify raw sequencing reads and file formats (FASTQ, SAM, VCF…) • Interpret Phred scores, read length, depth and coverage • Navigate a standard NGS analysis pipeline
Outline
Run FastQC and generate MultiQC summary reports • Detect adapters, low‑quality bases and duplication • Trim and filter reads using fastp / Trimmomatic • Compare raw vs cleaned reads and document QC metrics
Outline
Review alignment concepts and SAM/BAM statistics • Explore variant calling basics and VCF interpretation • Introduce RNA‑Seq count data and differential expression overview • Visualise results with IGV and simple plotting
e-Certificate and e-Marksheet issued on successful completion.