Detect antimicrobial-resistance genes from sequence data with BLAST.
Antimicrobial Resistance (AMR)
Module-by-module breakdown of Hands-On AMR Course: Detecting Resistance Genes Using BLAST & NCBI, from foundations to a certified capstone project.
Resources
โข GenBank, RefSeq, SRA and BioSample, and what each record actually guarantees
โข Retrieving sequences and metadata programmatically with E-utilities
โข Record quality: annotation errors and misidentified submissions
BLAST
โข BLAST flavours and choosing between blastn, blastp and tblastn
โข E-value, bit score, identity and coverage, and why identity alone misleads
โข Building and searching a local database for reproducible results
AMR Databases
โข CARD, ResFinder and AMRFinderPlus, and their differing curation philosophies
โข Acquired resistance genes versus chromosomal resistance mutations
โข Thresholds for calling a gene present, and disagreement between tools
Interpretation
โข Why a detected gene does not guarantee expressed resistance
โข Correlating predictions with phenotypic susceptibility testing
โข Mobile genetic elements, plasmids and transmissibility
Reporting
โข Scripting the workflow so a result can be regenerated
โข Version pinning of databases, which change underneath you
โข Reporting conventions for surveillance and publication
e-Certificate and e-Marksheet issued on successful completion.