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DSTC-01548 Online (e-LMS) Foundation

Hands-On AMR Course: Detecting Resistance Genes Using BLAST & NCBI

by - DSTC

Detect antimicrobial-resistance genes from sequence data with BLAST.

โ˜…โ˜…โ˜…โ˜…โ˜… Be the first to review โ€ข 3 Days ยท 4.5 hrs โ€ข e-Certificate Included
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From โ‚น2,500 + GST

๐Ÿ“š Syllabus & Course Curriculum

Antimicrobial Resistance (AMR)

Module-by-module breakdown of Hands-On AMR Course: Detecting Resistance Genes Using BLAST & NCBI, from foundations to a certified capstone project.

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Resources

โ€ข GenBank, RefSeq, SRA and BioSample, and what each record actually guarantees
โ€ข Retrieving sequences and metadata programmatically with E-utilities
โ€ข Record quality: annotation errors and misidentified submissions

BLAST

โ€ข BLAST flavours and choosing between blastn, blastp and tblastn
โ€ข E-value, bit score, identity and coverage, and why identity alone misleads
โ€ข Building and searching a local database for reproducible results

AMR Databases

โ€ข CARD, ResFinder and AMRFinderPlus, and their differing curation philosophies
โ€ข Acquired resistance genes versus chromosomal resistance mutations
โ€ข Thresholds for calling a gene present, and disagreement between tools

Interpretation

โ€ข Why a detected gene does not guarantee expressed resistance
โ€ข Correlating predictions with phenotypic susceptibility testing
โ€ข Mobile genetic elements, plasmids and transmissibility

Reporting

โ€ข Scripting the workflow so a result can be regenerated
โ€ข Version pinning of databases, which change underneath you
โ€ข Reporting conventions for surveillance and publication

Earn government-registered certification in Hands-On AMR Course: Detecting Resistance Genes Using BLAST & NCBI

e-Certificate and e-Marksheet issued on successful completion.

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Scholar Registration

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