📚 Syllabus & Course Curriculum
Data Science & Analytics
Module-by-module breakdown of Comet Assay to Study Antimutagenic Activity of Different Compounds, from foundations to a certified capstone project.
Principle
• Single cell gel electrophoresis and the migration of damaged DNA
• Alkaline against neutral versions, and the lesion types each detects
• Strand breaks and alkali-labile sites, not mutation — the distinction matters
Protocol
• Cell preparation, viability checks and the agarose embedding steps
• Lysis, unwinding and electrophoresis conditions and their sensitivity
• Neutralisation, staining and the slide handling that avoids artefacts
Scoring
• Tail moment, percentage DNA in tail and tail length compared
• Image analysis software and blinded scoring to prevent bias
• Cell number per slide and the distribution-based analysis it requires
Design
• Pre-treatment, co-treatment and post-treatment designs and what each demonstrates
• Positive and negative controls, and the cytotoxicity threshold that invalidates results
• Dose-response and separating antioxidant effect from direct interaction
Standards
• OECD Test Guideline 489 expectations for the in vivo assay
• Historical control data and inter-laboratory variability
• Complementary assays such as micronucleus and Ames for a defensible claim
Earn government-registered certification in Comet Assay to Study Antimutagenic Activity of Different Compounds
e-Certificate and e-Marksheet issued on successful completion.
