A bioinformatics approach to antimicrobial resistance and gene transfer.
Antimicrobial Resistance (AMR)
Module-by-module breakdown of Bioinformatics Approach to Antimicrobial Resistance & HGT, from foundations to a certified capstone project.
Isolates
โข Illumina and Nanopore data, and why long reads resolve plasmids
โข Assembly with SPAdes or Flye and assessing quality with QUAST and BUSCO
โข Species confirmation and contamination screening before anything else
Resistome
โข ResFinder, CARD-RGI and AMRFinderPlus and their different curation choices
โข Acquired genes against chromosomal point mutations conferring resistance
โข Genotype-to-phenotype concordance and where prediction reliably fails
Mobilome
โข Plasmid reconstruction, replicon typing and MOB typing
โข Integrons, transposons and insertion sequences flanking resistance genes
โข Chromosomal against plasmid location and its epidemiological weight
Phylogeny
โข MLST, cgMLST and core genome SNP analysis for relatedness
โข Recombination masking before tree building with Gubbins
โข Distinguishing clonal expansion from independent horizontal acquisition
Reporting
โข Submission to public databases and the metadata standards expected
โข Outbreak reporting and the SNP thresholds used, with their limits
โข Writing a genomic AMR report that a microbiologist can act on
e-Certificate and e-Marksheet issued on successful completion.