📚 Syllabus & Course Curriculum
Bioinformatics & Computational Biology
Module-by-module breakdown of Bacterial Comparative Genomics, from foundations to a certified capstone project.
Outline
Install and configure genomics tools using Conda package management • Obtain and manage raw sequencing reads from public repositories • Perform quality control assessment and adapter trimming of raw data • Execute de novo genome assembly using SPAdes assembler
Outline
Polish draft assemblies to correct errors and improve consensus accuracy using Pilon • Reorder and scaffold contigs against reference genomes using RagTag • Perform multi-locus sequence typing for strain identification and epidemiology • Annotate draft genomes comprehensively using Prokka pipeline
Outline
Identify antimicrobial resistance genes using Abricate screening tool • Conduct pangenome analysis to reveal core and accessory genome dynamics • Explore comparative genomics webservers including BV-BRC for integrated analysis
Outline
Reconstruct phylogenomic relationships from whole-genome comparisons • Analyze genetic diversity and population structure across bacterial isolates • Interpret horizontal gene transfer events and mobile genetic elements
Outline
Characterize resistance mechanisms using curated antimicrobial databases • Predict virulence factors and pathogenicity islands from genomic data • Correlate genotype with phenotype for clinical and environmental isolates
Outline
Build pangenome matrices using Roary and related pangenome tools • Visualize pangenome dynamics through interactive graphical representations • Quantify gene presence-absence patterns and their biological significance
Outline
Navigate BV-BRC comprehensive bacterial bioinformatics resource center • Integrate multi-omics data for systems-level bacterial analysis • Leverage cloud-based infrastructure for large-scale comparative studies
Earn government-registered certification in Bacterial Comparative Genomics
e-Certificate and e-Marksheet issued on successful completion.
