Programme Parameters
Foundation
3 Days (4.5 Hrs)
Online (e-LMS)
• No prior experience required — basic computer literacy is sufficient.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
Gene editing using CRISPR/Cas9 has become a revolutionary tool in genetic research, enabling precise modifications at the genome level. Induced pluripotent stem cells (iPSCs), generated from adult cells, hold immense potential for regenerative medicine, disease modeling, and drug discovery. CRISPR/Cas9 allows for the precise targeting and modification of specific genes within iPSCs, enabling the creation of disease models and providing insights into the genetic basis of diseases like neurodegenerative disorders, cancer, and genetic syndromes. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
Gene editing using CRISPR/Cas9 has become a revolutionary tool in genetic research, enabling precise modifications at the genome level. Induced pluripotent stem cells (iPSCs), generated from adult cells, hold immense potential for regenerative medicine, disease modeling, and drug discovery. CRISPR/Cas9 allows for the precise targeting and modification of specific genes within iPSCs, enabling the creation of disease models and providing insights into the genetic basis of diseases like neurodegenerative disorders, cancer, and genetic syndromes.
📋 Course Objectives
1. Apply biotechnology methods to authentic research and industry problems.
2. Assemble a documented case study that evidences your applied capability.
👥 Who Should Enroll?
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
🚀 Key Learning Outcomes
• A demonstrable biotechnology project for your research or industry portfolio.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
iPSC Handling and Baseline Quality Control
• Feeder-free maintenance, passaging technique and colony morphology assessment
• Pluripotency verification and genomic stability monitoring by karyotype or SNP array
• Establishing a baseline before editing so later changes are attributable
Nuclease Selection and Guide RNA Design
• PAM constraints and on-target activity scoring
• Off-target prediction with Cas-OFFinder and CFD scoring, and the limits of prediction
• High-fidelity variants: eSpCas9 and SpCas9-HiFi
• When base editing or prime editing is the better tool than a double-strand break
Editing Strategy and Repair Pathway Control
• RNP nucleofection versus plasmid and viral delivery in iPSCs
• NHEJ knockout versus HDR knock-in: choosing the repair outcome
• Donor template design: ssODN versus AAV, homology arm length, silent PAM mutation
• Cell-cycle timing and HDR enhancement, with realistic efficiency expectations
Clonal Isolation and Genotype Confirmation
• Single-cell cloning and surviving the bottleneck: ROCK inhibition and plating density
• Editing efficiency deconvolution from Sanger traces using ICE or TIDE
• Zygosity determination and detection of large deletions that PCR can miss
• NGS amplicon validation of the final clone
Off-Target and Genomic Integrity Assessment
• Empirical off-target discovery: GUIDE-seq, CIRCLE-seq and DISCOVER-seq
• Copy-number and karyotype reassessment after clonal expansion
• p53 pathway selection pressure and its implications for edited iPSC lines
Isogenic Disease Modelling and Reporting
• Directed differentiation of edited lines to the relevant lineage
• Isogenic pairs: designing the comparison so the phenotype is attributable to the edit
• Functional assays and phenotype validation
• Reporting standards and line registration for reproducibility
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | Benchling |
| Covered Tool / Platform | CRISPOR |
| Covered Tool / Platform | Cas-OFFinder |
| Covered Tool / Platform | SnapGene |
| Covered Tool / Platform | Addgene |
| Covered Tool / Platform | NCBI Primer-BLAST |
Frequently Asked Questions
Scholar Feedback & Reviews
Based on 0 scholar submissions
Rating Breakdown
No verified reviews published yet. Be the first to share your academic experience.
Leave Scholar Feedback
Your rating will help prospective scholars. Ratings below 3 stars are routed privately to the faculty mentor for immediate response.
