Programme Parameters
Advanced Postgrad
3 Days (4.5 Hrs)
Online (e-LMS)
• Prior working knowledge of the field and comfort with core tools and quantitative reasoning.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
This 3-day hands-on course provides an in-depth exploration of multiplex digital PCR (dPCR), a revolutionary technique for the absolute quantification of nucleic acids. Participants will delve into the core principles of dPCR, learn to design robust multiplex assays, effectively troubleshoot experiments, and analyze real-world data using cutting-edge simulation tools. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
This 3-day hands-on course provides an in-depth exploration of multiplex digital PCR (dPCR), a revolutionary technique for the absolute quantification of nucleic acids. Participants will delve into the core principles of dPCR, learn to design robust multiplex assays, effectively troubleshoot experiments, and analyze real-world data using cutting-edge simulation tools.
📋 Course Objectives
1. Translate biotechnology theory into practical, reproducible analysis.
2. Build a defensible project you can showcase to supervisors, reviewers, or employers.
👥 Who Should Enroll?
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
🚀 Key Learning Outcomes
• A demonstrable biotechnology project for your research or industry portfolio.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Mastering dPCR Fundamentals
Introduce dPCR principles and advantages over qPCR techniques. • Explore the intricacies of droplet and chip-based dPCR systems. • Understand the crucial concept of absolute quantification in molecular biology. • Review diverse applications in oncology, virology, and plant biotechnology.
Advanced Multiplexing Design & Optimization
Develop effective primer and probe design strategies for multiplex assays. • Implement techniques to prevent dimer formation and spectral overlap issues. • Optimize multiplex assay parameters for peak performance and accuracy. • Simulate multiplex dPCR setups using specialized software tools.
Practical dPCR Experimentation & Data Mastery
Execute a complete multiplex dPCR experiment, including instrument calibration and loading. • Perform endpoint fluorescence reading and comprehensive data analysis. • Analyze and interpret complex dPCR results accurately. • Troubleshoot common experimental issues and apply effective solutions.
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | dPCR instruments |
| Covered Tool / Platform | Software simulation tools |
Frequently Asked Questions
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