Programme Parameters
Graduate / Intermediate
4 Weeks (40 Hrs)
Online (e-LMS)
• A basic understanding of the subject area and fundamental programming or scientific concepts.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
This program offers a comprehensive exploration of the CRISPR-Cas system, from its natural origins to its revolutionary applications in genetic engineering. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
This program offers a comprehensive exploration of the CRISPR-Cas system, from its natural origins to its revolutionary applications in genetic engineering.
📋 Course Objectives
1. Apply biotechnology methods to authentic research and industry problems.
2. Produce a reproducible, portfolio-ready project you can cite in a thesis, paper, or job application.
👥 Who Should Enroll?
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
🚀 Key Learning Outcomes
• A portfolio-grade biotechnology deliverable you can defend and extend.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Bacterial Adaptive Immunity
• Repeat-spacer arrays and how they were recognised as an immune memory
• Adaptation, expression and interference as the three phases of defence
• PAM recognition and the self versus non-self discrimination problem
Classes, Types and Effectors
• Class 1 multi-subunit Cascade systems against Class 2 single-effector systems
• Cas9, Cas12 and Cas13 and their different cut chemistries and substrates
• Collateral cleavage in Cas12a and Cas13 and its diagnostic exploitation
Phage Counter-Defence
• Anti-CRISPR proteins and the mechanisms by which they disable effectors
• The evolutionary arms race shaping spacer acquisition rates
• Nucleus-like compartments and other phage strategies for evading detection
What Happens After the Cut
• NHEJ, MMEJ and HDR outcomes and their very different frequencies
• Indel spectra and why a knockout population is never uniform
• Base and prime editing as approaches that avoid the double-strand break
From Mechanism to Tool
• Why understanding the native system predicts engineering behaviour
• Off-target activity and the assays that measure it, including GUIDE-seq
• Diagnostics built on collateral cleavage, such as SHERLOCK and DETECTR
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | Benchling |
| Covered Tool / Platform | CRISPOR |
| Covered Tool / Platform | Cas-OFFinder |
| Covered Tool / Platform | SnapGene |
| Covered Tool / Platform | Addgene |
| Covered Tool / Platform | NCBI Primer-BLAST |
Frequently Asked Questions
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