Programme Parameters
Graduate / Intermediate
3 Days (4.5 Hrs)
Online (e-LMS)
• A basic understanding of the subject area and fundamental programming or scientific concepts.
• A laptop or desktop with a stable internet connection.
• Willingness to complete assignments and the capstone project.
About This Course
This 3‑day beginner‑friendly program introduces the fundamentals of Next‑Generation Sequencing (NGS) data processing and biological interpretation. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
🎯 Program Aim
This 3‑day beginner‑friendly program introduces the fundamentals of Next‑Generation Sequencing (NGS) data processing and biological interpretation.
📋 Course Objectives
1. Apply biotechnology methods to authentic research and industry problems.
2. Build a defensible project you can showcase to supervisors, reviewers, or employers.
👥 Who Should Enroll?
• Master's and senior undergraduate students specializing in biotechnology
• R&D engineers and working professionals applying biotechnology in industry
• Academics and educators building research or teaching capacity in biotechnology
🚀 Key Learning Outcomes
• A portfolio-grade biotechnology deliverable you can defend and extend.
• A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
💎 What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Day 1 – Introduction to NGS and Sequencing Data Fundamentals
Explore major NGS platforms and applications • Identify raw sequencing reads and file formats (FASTQ, SAM, VCF…) • Interpret Phred scores, read length, depth and coverage • Navigate a standard NGS analysis pipeline
Day 2 – Quality Control, Read Cleaning and Preprocessing
Run FastQC and generate MultiQC summary reports • Detect adapters, low‑quality bases and duplication • Trim and filter reads using fastp / Trimmomatic • Compare raw vs cleaned reads and document QC metrics
Day 3 – Basic NGS Workflow Interpretation & Biological Reporting
Review alignment concepts and SAM/BAM statistics • Explore variant calling basics and VCF interpretation • Introduce RNA‑Seq count data and differential expression overview • Visualise results with IGV and simple plotting
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | FastQC |
| Covered Tool / Platform | MultiQC |
| Covered Tool / Platform | fastp |
| Covered Tool / Platform | Trimmomatic |
| Covered Tool / Platform | SAMtools |
| Covered Tool / Platform | IGV |
| Covered Tool / Platform | Google Colab |
| Covered Tool / Platform | Google Sheets |
| Covered Tool / Platform | FASTQ Viewer |
Frequently Asked Questions
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