Programme Parameters
Advanced Postgrad
2 Days (3 Hrs)
Online (e-LMS)
β’ Prior working knowledge of the field and comfort with core tools and quantitative reasoning.
β’ A laptop or desktop with a stable internet connection.
β’ Willingness to complete assignments and the capstone project.
About This Course
Small interfering RNAs (siRNAs) have revolutionized molecular biology by providing precise gene silencing solutions. This 2-day hands-on course by DSTC delves into the core principles of RNA interference (RNAi), guiding participants through the understanding, design, and analysis of siRNA molecules using cutting-edge bioinformatics tools. Every participant receives a verified e-Certificate and e-Marksheet from the Deep Science & Technology Consortium.
π― Program Aim
Small interfering RNAs (siRNAs) have revolutionized molecular biology by providing precise gene silencing solutions. This 2-day hands-on course by DSTC delves into the core principles of RNA interference (RNAi), guiding participants through the understanding, design, and analysis of siRNA molecules using cutting-edge bioinformatics tools.
π Course Objectives
1. Translate biotechnology theory into practical, reproducible analysis.
2. Produce a reproducible, portfolio-ready project you can cite in a thesis, paper, or job application.
π₯ Who Should Enroll?
β’ Master's and senior undergraduate students specializing in biotechnology
β’ R&D engineers and working professionals applying biotechnology in industry
β’ Academics and educators building research or teaching capacity in biotechnology
π Key Learning Outcomes
β’ A portfolio-grade biotechnology deliverable you can defend and extend.
β’ A verified e-Certificate of competency and e-Marksheet from the Deep Science & Technology Consortium.
π What You'll Gain
Live & Recorded Sessions
Lifetime access to class recordingse-Certificate on Completion
Cryptographically verified credentialPost-Programme Support
Direct access to mentors & councilHands-On Experience
Notebooks, real-world code & datasetsCurriculum Outline
Introduction to RNAi & siRNA Fundamentals
Explore the foundational concepts of RNA interference (RNAi) and small interfering RNAs (siRNA). β’ Identify key applications of siRNA in therapeutics and gene regulation. β’ Understand the basic structure and functional rules governing siRNAs.
Differentiating RNAi Molecules
Distinguish between siRNA, microRNA (miRNA), and short hairpin RNA (shRNA) based on structure and mechanism. β’ Analyze the unique characteristics and roles of each RNAi molecule. β’ Discuss potential off-target effects and the importance of GC content in siRNA design.
Understanding RNA Secondary Structures
Introduce the principles of RNA secondary structures, including base pairing, stem-loops, and hairpins. β’ Interpret different RNA structure formats. β’ Lay the groundwork for advanced RNA folding analysis.
Advanced RNA Structure Prediction & Analysis
Utilize leading RNA secondary structure prediction tools such as RNAfold (ViennaRNA), Mfold, and RNAstructure. β’ Practice interpreting Free Energy (ΞG) values to assess RNA stability. β’ Generate and analyze structure ensembles for comprehensive RNA folding insights.
Practical siRNA Sequence Design
Apply theoretical knowledge to hands-on siRNA sequence design exercises. β’ Leverage professional bioinformatics platforms like Genscript for effective siRNA creation. β’ Evaluate designed siRNA sequences for optimal efficiency and specificity.
Technical Specifications
| Parameter | Requirement |
|---|---|
| Covered Tool / Platform | RNAfold (ViennaRNA) |
| Covered Tool / Platform | Mfold |
| Covered Tool / Platform | RNAstructure |
| Covered Tool / Platform | Genscript |
| Covered Tool / Platform | Free Energy (ΞG) analysis |
| Covered Tool / Platform | Structure Ensemble Generation |
Frequently Asked Questions
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