The Silent Pandemic of Antimicrobial Resistance
Antimicrobial Resistance (AMR) poses one of the greatest global health threats of the 21st century. High-throughput shotgun metagenomic sequencing allows researchers to profile the complete antibiotic resistome within clinical, environmental, and agricultural samples without culture-dependent limitations.
Metagenomic AMR Analytical Pipeline
- Quality Control & Host Decontamination: Trimmomatic / FastP quality filtering followed by host DNA subtraction using Bowtie2.
- Resistome Profiling: Mapping reads against curated resistance databases (CARD, ResFinder, ARDB).
- Horizontal Gene Transfer (HGT) Detection: Identifying plasmid-borne resistance determinants and mobile genetic elements (MGEs) using assembly graph analysis.
🧬 Master Metagenomic AMR Analytics
Learn hands-on pipelines to track antibiotic resistance genes, plasmid conjugation, and public health surveillance analytics.
Frequently Asked Questions
What is the difference between targeted PCR and metagenomic resistome profiling?
Targeted PCR only identifies pre-selected known resistance genes, whereas metagenomic sequencing discovers novel resistance variants, mutations, and plasmid-associated mobilomes across whole microbial communities.